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Top of pageAbstractIn epithelial tissues, the lineage relationship between normal progenitor cells and cell type(s) of origin for cancer has been poorly understood. Here we show that a known regulator of prostate epithelial differentiation, the homeobox gene Nkx3 1, marks a stem cell population that functions during prostate regeneration. Genetic lineage marking demonstrates that rare luminal cells that express Nkx3 1 in the absence of testicular androgens (castration resistant Nkx3 1 expressing cells, CARNs) are bipotential and can self renew in vivo, and single cell transplantation assays show that CARNs can reconstitute prostate ducts in renal grafts.

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